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Proceedings Paper

Light-sheet optimization for microscopy
Author(s): Dean Wilding; Paolo Pozzi; Oleg Soloviev; Gleb Vdovin; Michel Verhaegen
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Paper Abstract

Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.

Paper Details

Date Published: 15 March 2016
PDF: 8 pages
Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 971718 (15 March 2016); doi: 10.1117/12.2210912
Show Author Affiliations
Dean Wilding, Delft Univ. of Technology (Netherlands)
Paolo Pozzi, Delft Univ. of Technology (Netherlands)
Oleg Soloviev, Delft Univ. of Technology (Netherlands)
Flexible Optical B.V. (Netherlands)
Gleb Vdovin, Delft Univ. of Technology (Netherlands)
Flexible Optical B.V. (Netherlands)
Michel Verhaegen, Delft Univ. of Technology (Netherlands)


Published in SPIE Proceedings Vol. 9717:
Adaptive Optics and Wavefront Control for Biological Systems II
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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