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Proceedings Paper

Laser spectrofluorometry of humic substances conformational transformation in water
Author(s): Nicolai A. Nemkovich; Anna P. Golovach; Alexander S. Kozlovsky; Ivan I. Lishtvan; Anatoly Nikolaevich Rubinov
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Paper Abstract

Conformational transformation of dissolved humic substances induced by acidic and basic additives in water has been investigated by automated subnanosecond laser spectrofluorimeter and fluorescent probe method. The equilibrium constants for binding of rhodamine 6G to dissolved humic substances in water have been determined by a fluorescence quenching method. It is shown that the conformational structure of humic compounds in water depends on the intra- and intermolecular interactions. Spectral and viscosimetrical data in pH region 1.5-12.0 are interpreted in terms of conformational transformation of dissolved humic substances from a rigid globule to a flexible chain.

Paper Details

Date Published: 21 September 1995
PDF: 9 pages
Proc. SPIE 2503, Air Toxics and Water Monitoring, (21 September 1995); doi: 10.1117/12.221091
Show Author Affiliations
Nicolai A. Nemkovich, B. I. Stepanov Institute of Physics (Belarus)
Anna P. Golovach, Institute for Problems of the Use of Natural Resources and Ecology (Belarus)
Alexander S. Kozlovsky, B. I. Stepanov Institute of Physics (Belarus)
Ivan I. Lishtvan, Institute for Problems of the Use of Natural Resources and Ecology (Belarus)
Anatoly Nikolaevich Rubinov, B. I. Stepanov Institute of Physics (Belarus)


Published in SPIE Proceedings Vol. 2503:
Air Toxics and Water Monitoring

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