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Proceedings Paper

The study on RBC characteristic in paroxysmal nocturnal hemoglobinuria (PNH) patients using common path interferometric quantitative phase microscopy
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Paper Abstract

We have studied the RBC membrane properties between a normal RBC and a RBC in Paroxysrnal nocturnal hemoglobinuria (PNH) patient using common path interferometric quantitative phase microscopy (CPIQPM). CPIQPM system has provided the subnanometer optical path length sensitivity on a millisecond. We have measured the dynamic thickness fluctuations of a normal RBC membrane and a RBC membrane in PNH patient over the whole cell surface with CPIQPM. PNH is a rare and serious disease of blood featured by destruction of red blood cells (RBCs). This destruction happens since RBCs show the defect of protein which protects RBCs from the immune system. We have applied CPIQPM to study the characteristic of RBC membrane in PNH patient. We have shown the morphological shape, volume, and projected surface for both different RBC types. The results have showed both RBCs had the similar shape with donut, but membrane fluctuations in PNH patient was shown to reveal the difference of temporal properties compared with a normal RBC. In order to demonstrate the practical tool of the CPIQPM technique, we have also obtained the time series thickness fluctuation outside a cell.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971819 (9 March 2016); doi: 10.1117/12.2210873
Show Author Affiliations
Byung Jun Park, Osong Medical Innovation Foundation (Korea, Republic of)
Youngjae Won, Osong Medical Innovation Foundation (Korea, Republic of)
Byungyeon Kim, Osong Medical Innovation Foundation (Korea, Republic of)
Seungrag Lee, Osong Medical Innovation Foundation (Korea, Republic of)


Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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