Share Email Print
cover

Proceedings Paper

Functional optical coherence tomography for high-resolution mapping of cilia beat frequency in the mouse oviduct in vivo
Author(s): Shang Wang; Jason C. Burton; Richard R. Behringer; Irina V. Larina
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Since mouse is a superior model for genetic analysis of human disorders, reproductive studies in mice have significant implications on further understanding of fertility and infertility in humans. Fertilized oocytes are transported through the reproductive tract by motile cilia lining the lumen of the oviduct as well as by oviduct contractions. While the role of cilia is well recognized, ciliary dynamics in the oviduct is not well understood, largely owing to the lack of live imaging approaches. Here, we report in vivo micro-scale mapping of cilia and cilia beat frequency (CBF) in the mouse oviduct using optical coherence tomography (OCT). This functional imaging method is based on spectral analysis of the OCT speckle variations produced by the beat of cilia in the oviduct, which does not require exogenous contrast agents. Animal procedures similar to the ones used for production of transgenic mice are utilized to expose the reproductive organs for imaging in anesthetized females. In this paper, we first present in vivo structural imaging of the mouse oviduct capturing the oocyte and the preimplantation embryo and then show the result of depth-resolved high-resolution CBF mapping in the ampulla of the live mouse. These data indicate that this structural and functional OCT imaging approach can be a useful tool for a variety of live investigations of mammalian reproduction and infertility.

Paper Details

Date Published: 29 February 2016
PDF: 5 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893R (29 February 2016); doi: 10.1117/12.2210758
Show Author Affiliations
Shang Wang, Baylor College of Medicine (United States)
Jason C. Burton, Baylor College of Medicine (United States)
Richard R. Behringer, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Irina V. Larina, Baylor College of Medicine (United States)


Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

© SPIE. Terms of Use
Back to Top