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Proceedings Paper

Live dynamic OCT imaging of cardiac structure and function in mouse embryos with 43 Hz direct volumetric data acquisition
Author(s): Shang Wang; Manmohan Singh; Andrew L. Lopez; Chen Wu; Raksha Raghunathan; Alexander Schill; Jiasong Li; Kirill V. Larin; Irina V. Larina
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Paper Abstract

Efficient phenotyping of cardiac dynamics in live mouse embryos has significant implications on understanding of early mammalian heart development and congenital cardiac defects. Recent studies established optical coherence tomography (OCT) as a powerful tool for live embryonic heart imaging in various animal models. However, current four-dimensional (4D) OCT imaging of the beating embryonic heart largely relies on gated data acquisition or postacquisition synchronization, which brings errors when cardiac cycles lack perfect periodicity and is time consuming and computationally expensive. Here, we report direct 4D OCT imaging of the structure and function of cardiac dynamics in live mouse embryos achieved by employing a Fourier domain mode-locking swept laser source that enables ~1.5 MHz A-line rate. Through utilizing both forward and backward scans of a resonant mirror, we obtained a ~6.4 kHz frame rate, which allows for a direct volumetric data acquisition speed of ~43 Hz, around 20 times of the early-stage mouse embryonic heart rate. Our experiments were performed on mouse embryos at embryonic day 9.5. Time-resolved 3D cardiodynamics clearly shows the heart structure in motion. We present analysis of cardiac wall movement and its velocity from the primitive atrium and ventricle. Our results suggest that the combination of ultrahigh-speed OCT imaging with live embryo culture could be a useful embryonic heart phenotyping approach for mouse mutants modeling human congenital heart diseases.

Paper Details

Date Published: 9 March 2016
PDF: 4 pages
Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 971603 (9 March 2016); doi: 10.1117/12.2210751
Show Author Affiliations
Shang Wang, Baylor College of Medicine (United States)
Manmohan Singh, Univ. of Houston (United States)
Andrew L. Lopez, Baylor College of Medicine (United States)
Chen Wu, Univ. of Houston (United States)
Raksha Raghunathan, Univ. of Houston (United States)
Alexander Schill, Univ. of Houston (United States)
Jiasong Li, Univ. of Houston (United States)
Kirill V. Larin, Baylor College of Medicine (United States)
Univ. of Houston (United States)
Samara State Aerospace Univ. (Russian Federation)
Irina V. Larina, Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 9716:
Optical Methods in Developmental Biology IV
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma, Editor(s)

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