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Proceedings Paper

Ultrastructural imaging and molecular modeling of live bacteria using soft x-ray contact microscopy with nanoseconds laser-plasma radiation
Author(s): Masataka Kado; Martin C. Richardson; Kai Gaebel; David Scott Torres; Jayshree Rajyaguru; Michael J. Muszynski
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Paper Abstract

X-ray images of the various live bacteria, such as Staphylococcus and Streptococcus, and micromolecule such as chromosomal DNA from Escherichis coli, and Lipopolysacchride from Burkholderia cepacia, are obtained with soft x-ray contact microscopy. A compact tabletop type glass laser system is used to produce x-rays from Al, Si, and Au targets. The PMMA photoresists are used to record x-ray images. An AFM (atomic force microscope) is used to reproduce the x-ray images from the developed photoresists. The performance of the 50nm spatial resolutions are achieved and images are able to be discussed on the biological view.

Paper Details

Date Published: 18 September 1995
PDF: 8 pages
Proc. SPIE 2523, Applications of Laser Plasma Radiation II, (18 September 1995); doi: 10.1117/12.220979
Show Author Affiliations
Masataka Kado, CREOL/Univ. of Central Florida (United States)
Martin C. Richardson, CREOL/Univ. of Central Florida (United States)
Kai Gaebel, CREOL/Univ. of Central Florida (United States)
David Scott Torres, CREOL/Univ. of Central Florida (United States)
Jayshree Rajyaguru, Arnold Palmer Hospital for Children and Women (United States)
Michael J. Muszynski, Arnold Palmer Hospital for Children and Women (United States)


Published in SPIE Proceedings Vol. 2523:
Applications of Laser Plasma Radiation II
Martin C. Richardson; George A. Kyrala, Editor(s)

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