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Proceedings Paper

X-ray contact microscopy using a plasma source generated by long and short (120-ns and 10-ns) excimer laser pulses
Author(s): Robin A. Cotton; Sarah Bollanti; Paolo Di Lazzaro; Francesco Flora; Nicola Lisi; Tommaso Letardi; Libero Palladino; Armando Reale; Dimitri Batani; Aldo Conti; A. Mauri; M. Moret; Lucia Reale; Patrizia Albertano; Antonio Grilli
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Paper Abstract

Soft x-ray contact microscopy (SXCM), using a pulsed x-ray source, offers the possibility of imaging the ultrastructure of living biological systems at sub-50nm resolution. We have developed a pulsed plasma x-ray source for this application, generated by the large volume XeCl laser 'Hercules'. Various unstable optical resonator configurations were employed to achieve a high laser intensity to increase the conversion efficiency to 'water window' x-rays (280-530eV). Optimum plasma conditions for SXCM are discussed, including the effect of pulse duration on image resolution. Soft x-ray contact images of Chlamydomonas dysosmos (unicellular alga) and the cyanobacteria Leptolyngbya are shown. In addition, the potential of producing a 'movie film' of the development of x-ray images within the photoresist (acting as the recording medium) is discussed, following the resist development while viewing by atomic force microscopy.

Paper Details

Date Published: 18 September 1995
PDF: 10 pages
Proc. SPIE 2523, Applications of Laser Plasma Radiation II, (18 September 1995); doi: 10.1117/12.220977
Show Author Affiliations
Robin A. Cotton, ENEA Frascati (Italy)
Sarah Bollanti, ENEA Frascati (Italy)
Paolo Di Lazzaro, ENEA Frascati (Italy)
Francesco Flora, ENEA Frascati (Italy)
Nicola Lisi, ENEA Frascati (Italy)
Tommaso Letardi, ENEA Frascati (Italy)
Libero Palladino, Univ.L'Aquila (Italy)
Armando Reale, Univ.L'Aquila (Italy)
Dimitri Batani, Univ. di Milano (Italy)
Aldo Conti, Univ. di Milano (Italy)
A. Mauri, Univ. di Milano (Italy)
M. Moret, Univ. di Milano (Italy)
Lucia Reale, Instituto Superior Sanita (Italy)
Patrizia Albertano, Univ. di Roma (Italy)
Antonio Grilli, Insituto Nazionale de Fisica Nucleare (Italy)


Published in SPIE Proceedings Vol. 2523:
Applications of Laser Plasma Radiation II
Martin C. Richardson; George A. Kyrala, Editor(s)

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