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Proceedings Paper

Light-assisted drying (LAD) of small volume biologics: a comparison of two IR light sources
Author(s): Madison A. Young; Matthew Van Vorst; Gloria D. Elliott; Susan R. Trammell
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Paper Abstract

Protein therapeutics have been developed to treat diseases ranging from arthritis and psoriasis to cancer. A challenge in the development of protein-based drugs is maintaining the protein in the folded state during processing and storage. We are developing a novel processing method, light-assisted drying (LAD), to dehydrate proteins suspended in a sugar (trehalose) solution for storage at supra-zero temperatures. Our technique selectively heats the water in small volume samples using near-IR light to speed dehydration which prevents sugar crystallization that can damage embedded proteins. In this study, we compare the end moisture content (EMC) as a function of processing time of samples dried with two different light sources, Nd:YAG (1064 nm) and Thulium fiber (1850 nm) lasers. EMC is the ratio of water to dry weight in a sample and the lower the EMC the higher the possible storage temperature. LAD with the 1064 and 1850 nm lasers yielded 78% and 65% lower EMC, respectively, than standard air-drying. After 40 minutes of LAD with 1064 and 1850 nm sources, EMCs of 0.27±.27 and 0.15±.05 gH2O/gDryWeight were reached, which are near the desired value of 0.10 gH2O/gDryWeight that enables storage in a glassy state without refrigeration. LAD is a promising new technique for the preparation of biologics for anhydrous preservation.

Paper Details

Date Published: 7 March 2016
PDF: 5 pages
Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 97060F (7 March 2016); doi: 10.1117/12.2209676
Show Author Affiliations
Madison A. Young, Univ. of North Carolina at Charlotte (United States)
Matthew Van Vorst, Univ. of North Carolina at Charlotte (United States)
Gloria D. Elliott, Univ. of North Carolina at Charlotte (United States)
Susan R. Trammell, Univ. of North Carolina at Charlotte (United States)

Published in SPIE Proceedings Vol. 9706:
Optical Interactions with Tissue and Cells XXVII
E. Duco Jansen, Editor(s)

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