Share Email Print
cover

Proceedings Paper

Noninvasive imaging of multiple myeloma using near infrared fluorescent molecular probe
Author(s): Deep Hathi; Haiying Zhou; Alex Bollerman-Nowlis; Monica Shokeen; Walter J. Akers
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Multiple myeloma is a plasma cell malignancy characterized by monoclonal gammopathy and osteolytic bone lesions. Multiple myeloma is most commonly diagnosed in late disease stages, presenting with pathologic fracture. Early diagnosis and monitoring of disease status may improve quality of life and long-term survival for multiple myeloma patients from what is now a devastating and fatal disease. We have developed a near-infrared targeted fluorescent molecular probe with high affinity to the α4β1 integrin receptor (VLA-4)overexpressed by a majority of multiple myeloma cells as a non-radioactive analog to PET/CT tracer currently being developed for human diagnostics. A near-infrared dye that emits about 700 nm was conjugated to a high affinity peptidomimmetic. Binding affinity and specificity for multiple myeloma cells was investigated in vitro by tissue staining and flow cytometry. After demonstration of sensitivity and specificity, preclinical optical imaging studies were performed to evaluate tumor specificity in murine subcutaneous and metastatic multiple myeloma models. The VLA-4-targeted molecular probe showed high affinity for subcutaneous MM tumor xenografts. Importantly, tumor cells specific accumulation in the bone marrow of metastatic multiple myeloma correlated with GFP signal from transfected cells. Ex vivo flow cytometry of tumor tissue and bone marrow further corroborated in vivo imaging data, demonstrating the specificity of the novel agent and potential for quantitative imaging of multiple myeloma burden in these models.

Paper Details

Date Published: 22 April 2016
PDF: 6 pages
Proc. SPIE 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, 972304 (22 April 2016); doi: 10.1117/12.2209662
Show Author Affiliations
Deep Hathi, Washington Univ. School of Medicine in St. Louis (United States)
Haiying Zhou, Washington Univ. School of Medicine in St. Louis (United States)
Alex Bollerman-Nowlis, Washington Univ. School of Medicine in St. Louis (United States)
Monica Shokeen, Washington Univ. School of Medicine in St. Louis (United States)
Walter J. Akers, Washington Univ. School of Medicine in St. Louis (United States)


Published in SPIE Proceedings Vol. 9723:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top