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Proceedings Paper

Binding of the immunomodulatory drug Bz-423 to mitochondrial FoF1-ATP synthase in living cells by FRET acceptor photobleaching
Author(s): Ilka Starke; Kathryn M. Johnson; Jan Petersen; Peter Gräber; Anthony W. Opipari; Gary D. Glick; Michael Börsch
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Paper Abstract

Bz-423 is a promising new drug for treatment of autoimmune diseases. This small molecule binds to subunit OSCP of the mitochondrial enzyme FoF1-ATP synthase and modulates its catalytic activities. We investigate the binding of Bz-423 to mitochondria in living cells and how subunit rotation in FoF1-ATP synthase, i.e. the mechanochemical mechanism of this enzyme, is affected by Bz-423. Therefore, the enzyme was marked selectively by genetic fusion with the fluorescent protein EGFP to the C terminus of subunit γ. Imaging the threedimensional arrangement of mitochondria in living yeast cells was possible at superresolution using structured illumination microscopy, SIM. We measured uptake and binding of a Cy5-labeled Bz-423 derivative to mitochondrial FoF1-ATP synthase in living yeast cells using FRET acceptor photobleaching microscopy. Our data confirmed the binding of Cy5-labeled Bz-423 to the top of the F1 domain of the enzyme in mitochondria of living Saccharomyces cerevisiae cells.

Paper Details

Date Published: 14 March 2016
PDF: 11 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120P (14 March 2016); doi: 10.1117/12.2209645
Show Author Affiliations
Ilka Starke, Jena Univ. Hospital, Friedrich Schiller Univ. (Germany)
Albert-Ludwigs-Univ. (Germany)
Kathryn M. Johnson, Univ. of Michigan (United States)
Jan Petersen, Albert-Ludwigs-Univ. (Germany)
Monash Univ. (Australia)
Peter Gräber, Albert-Ludwigs-Univ. (Germany)
Anthony W. Opipari, Univ. of Michigan (United States)
Gary D. Glick, Univ. of Michigan (United States)
Michael Börsch, Jena Univ. Hospital, Friedrich Schiller Univ. (Germany)
Ctr. for Medical Optics and Photonics (Germany)
Abbe Ctr. of Photonics (Germany)

Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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