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Proceedings Paper

A study on the characteristics of the Analog Mean Delay (AMD) method for high-speed Fluorescence Lifetime Imaging Microscopy (FLIM)
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Paper Abstract

We present a study on the characteristics of the AMD method. We have demonstrated that the photon economy of the AMD method is not degraded for longer lifetimes even when the applied integration window size is increased. By an extension of MCS, the photon economy with respect to different designs of the Gaussian low-pass filter (GLPF) used in the AMD setup was also studied. When a GLPF with the highest cutoff frequency of 100 MHz is applied, the most effective photon economy performance is achieved for lifetimes of 1, 3.2, 5, and 8 ns.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 972014 (7 March 2016); doi: 10.1117/12.2209468
Show Author Affiliations
Byungyeon Kim, Osong Medical Innovation Foundation (Korea, Republic of)
Byungjun Park, Osong Medical Innovation Foundation (Korea, Republic of)
Seungrag Lee, Osong Medical Innovation Foundation (Korea, Republic of)
Youngjae Won, Osong Medical Innovation Foundation (Korea, Republic of)


Published in SPIE Proceedings Vol. 9720:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management
Kevin K. Tsia; Keisuke Goda, Editor(s)

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