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Visualization of tumor vascular reactivity in response to respiratory challenges by optical coherence tomography (Conference Presentation)
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Paper Abstract

We previously reported the potential of using vascular reactivity during respiratory challenges as a marker to predict the response of breast tumor to chemotherapy in a rat model by using a continuous wave near-infrared spectroscopy. However, it cannot visualize how the vascular reactivity from tumor vessel can predict the tumor response to its treatment. In this study, we utilized a spectral domain optical coherence tomography (SD-OCT) system to visualize vascular reactivity of both tumor and normal vasculature during respiratory challenges in a mouse model. We adapted intensity based Doppler variance algorithm to draw angiogram from the ear of mouse (8-week-old Balb/c nu/nu). Animals were anesthetized using 1.5% isoflurane, and the body temperature was maintained by a heating pad. Inhalational gas was switched from air (10min) to 100% oxygen (10min), and a pulse oximeter was used to monitor arterial oxygen saturation and heart rate. OCT angiograms were acquired 5 min after the onset of each gas. The vasoconstriction effect of hyperoxic gas on vasculature was shown by subtracting an en-face image acquired during 100% oxygen from the image acquired during air inhalation. The quantitative change in the vessel diameter was measured from the en-face OCT images of the individual blood vessels. The percentage of blood vessel diameter reduction varied from 1% to 12% depending on arterial, capillary, or venous blood vessel. The vascular reactivity change during breast tumor progression and post chemotherapy will be monitored by OCT angiography.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893Y (27 April 2016); doi: 10.1117/12.2209439
Show Author Affiliations
Hoon Sup Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Songhyun Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Kiri Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Tae Joong Eom, Gwangju Institute of Science and Technology (Korea, Republic of)
Advanced Photonics Research Institute (Korea, Republic of)
Jae G. Kim, Gwangju Institute of Science and Technology (Korea, Republic of)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong; Justus F. Ilgner; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory; Laura Marcu; Haishan Zeng, Editor(s)

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