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Proceedings Paper

Unmixing chromophores in human skin with a 3D multispectral optoacoustic mesoscopy system
Author(s): Mathias Schwarz; Juan Aguirre; Dominik Soliman; Andreas Buehler; Vasilis Ntziachristos
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Paper Abstract

The absorption of visible light by human skin is governed by a number of natural chromophores: Eumelanin, pheomelanin, oxyhemoglobin, and deoxyhemoglobin are the major absorbers in the visible range in cutaneous tissue. Label-free quantification of these tissue chromophores is an important step of optoacoustic (photoacoustic) imaging towards clinical application, since it provides relevant information in diseases. In tumor cells, for instance, there are metabolic changes (Warburg effect) compared to healthy cells, leading to changes in oxygenation in the environment of tumors. In malignant melanoma changes in the absorption spectrum have been observed compared to the spectrum of nonmalignant nevi. So far, optoacoustic imaging has been applied to human skin mostly in single-wavelength mode, providing anatomical information but no functional information. In this work, we excited the tissue by a tunable laser source in the spectral range from 413-680 nm with a repetition rate of 50 Hz. The laser was operated in wavelengthsweep mode emitting consecutive pulses at various wavelengths that allowed for automatic co-registration of the multispectral datasets. The multispectral raster-scan optoacoustic mesoscopy (MSOM) system provides a lateral resolution of <60 μm independent of wavelength. Based on the known absorption spectra of melanin, oxyhemoglobin, and deoxyhemoglobin, three-dimensional absorption maps of all three absorbers were calculated from the multispectral dataset.

Paper Details

Date Published: 15 March 2016
PDF: 9 pages
Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 970855 (15 March 2016); doi: 10.1117/12.2209333
Show Author Affiliations
Mathias Schwarz, Technische Univ. München (Germany)
Helmholtz Zentrum München (Germany)
Juan Aguirre, Technische Univ. München (Germany)
Helmholtz Zentrum München (Germany)
Dominik Soliman, Technische Univ. München (Germany)
Helmholtz Zentrum München (Germany)
Andreas Buehler, Technische Univ. München (Germany)
Helmholtz Zentrum München (Germany)
Vasilis Ntziachristos, Technische Univ. München (Germany)
Helmholtz Zentrum München (Germany)


Published in SPIE Proceedings Vol. 9708:
Photons Plus Ultrasound: Imaging and Sensing 2016
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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