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Proceedings Paper

Imaging melanin cancer growth in-vivo using raster-scan optoacoustic mesoscopy (RSOM) at 50 MHz and 100 MHz
Author(s): Murad Omar; Mathias Schwarz; Dominik Soliman; Panagiotis Symvoulidis; Vasilis Ntziachristos
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Paper Abstract

We used raster-scan optoacoustic mesoscopy (RSOM) at 50 MHz, and at 100 MHz, to monitor tumor growth, and tumor angiogenesis, which is a central hallmark of cancer, in-vivo. In this study we compared the performance, and the effect of the 50 MHz, and the 100 MHz frequencies on the quality of the final image.
The system is based on a reflection-mode implementation of RSOM. The detectors used are custom made, ultrawideband, and spherically focused. The use of such detectors enables light coupling from the same side as the detector, thus reflection-mode. Light is in turn coupled using a fiber bundle, and the detector is raster scanned in the xy-plane. Subsequently, to retrieve small features, the raw data are reconstructed using a multi-bandwidth, beamforming reconstruction algorithm.
Comparison of the system performance at the different frequencies shows as expected a higher resolution in case of the 100 MHz detector compared to the 50 MHz. On the other hand the 50 MHz has a better SNR, can detect features from deeper layers, and has higher angular acceptance. Based on these characteristics the 50 MHz detector was mostly used. After comparing the performance we monitored the growth of B16F10 cells, melanin tumor, over the course of 9 days. We see correspondence between the optoacoustic measurements and the cryoslice validations. Additionally, in areas close to the tumor we see sprouting of new vessels, starting at day 4-5, which corresponds to tumor angiogenesis.

Paper Details

Date Published: 15 March 2016
PDF: 6 pages
Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97080S (15 March 2016); doi: 10.1117/12.2209298
Show Author Affiliations
Murad Omar, TU Muenchen (Germany)
Helmholtz Zentrum Muenchen (Germany)
Mathias Schwarz, TU Muenchen (Germany)
Helmholtz Zentrum Muenchen (Germany)
Dominik Soliman, TU Muenchen (Germany)
Helmholtz Zentrum Muenchen (Germany)
Panagiotis Symvoulidis, TU Muenchen (Germany)
Helmholtz Zentrum Muenchen (Germany)
Vasilis Ntziachristos, TU Muenchen (Germany)
Helmholtz Zentrum Muenchen (Germany)

Published in SPIE Proceedings Vol. 9708:
Photons Plus Ultrasound: Imaging and Sensing 2016
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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