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Proceedings Paper

High resolution quantitative phase imaging of live cells with constrained optimization approach
Author(s): Vimal Prabhu Pandiyan; Kedar Khare; Renu John
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Paper Abstract

Quantitative phase imaging (QPI) aims at studying weakly scattering and absorbing biological specimens with subwavelength accuracy without any external staining mechanisms. Use of a reference beam at an angle is one of the necessary criteria for recording of high resolution holograms in most of the interferometric methods used for quantitative phase imaging. The spatial separation of the dc and twin images is decided by the reference beam angle and Fourier-filtered reconstructed image will have a very poor resolution if hologram is recorded below a minimum reference angle condition. However, it is always inconvenient to have a large reference beam angle while performing high resolution microscopy of live cells and biological specimens with nanometric features. In this paper, we treat reconstruction of digital holographic microscopy images as a constrained optimization problem with smoothness constraint in order to recover only complex object field in hologram plane even with overlapping dc and twin image terms. We solve this optimization problem by gradient descent approach iteratively and the smoothness constraint is implemented by spatial averaging with appropriate size. This approach will give excellent high resolution image recovery compared to Fourier filtering while keeping a very small reference angle. We demonstrate this approach on digital holographic microscopy of live cells by recovering the quantitative phase of live cells from a hologram recorded with nearly zero reference angle.

Paper Details

Date Published: 9 March 2016
PDF: 10 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971803 (9 March 2016); doi: 10.1117/12.2209289
Show Author Affiliations
Vimal Prabhu Pandiyan, Indian Institute of Technology Hyderabad (India)
Kedar Khare, Indian Institute of Technology Delhi (India)
Renu John, Indian Institute of Technology Hyderabad (India)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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