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Proceedings Paper

Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study
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Paper Abstract

The mouse is a common model for studying developmental diseases. Different optical techniques have been developed to investigate mouse embryos, but each has its own set of limitations and restrictions. In this study, we imaged the same E9.5 mouse embryo with rotational imaging Optical Coherence Tomography (RI-OCT) and Selective Plane Illumination Microscopy (SPIM), and compared the two techniques. Results demonstrate that both methods can provide images with micrometer-scale spatial resolution. The RI-OCT technique was developed to increase imaging depth of OCT by performing traditional OCT imaging at multiple sides and co-registering the images. In SPIM, optical sectioning is achieved by illuminating the sample with a sheet of light. In this study, the images acquired from both techniques are compared with each other to evaluate the benefits and drawbacks of each technique for embryonic imaging. Since 3D stacks can be obtained by SPIM from different angles by rotating the sample, it might be possible to build a hybrid setup of two imaging modalities to combine the advantages of each technique.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160B (9 March 2016); doi: 10.1117/12.2209147
Show Author Affiliations
Chen Wu, Univ. of Houston (United States)
Shihao Ran, Univ. of Houston (United States)
Henry H. Le, Baylor College of Medicine (United States)
Manmohan Singh, Univ. of Houston (United States)
Irina V. Larina, Baylor College of Medicine (United States)
David Mayerich, Univ. of Houston (United States)
Mary E. Dickinson, Baylor College of Medicine (United States)
Kirill V. Larin, Univ. of Houston (United States)
Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 9716:
Optical Methods in Developmental Biology IV
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma, Editor(s)

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