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Proceedings Paper

Potential of mid IR spectroscopy in the rapid label free identification of skin malignancies
Author(s): Lena Kastl; Björn Kemper; Gavin R. Lloyd; Jayakrupakar Nallala; Nick Stone; Valery Naranjo; Francisco Penaranda; Jürgen Schnekenburger
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Paper Abstract

The rapid inspection of suspicious skin lesions for pathological cell types is the objective of optical point of care diagnostics technologies. A marker free fast diagnosis of skin malignancies would overcome the limitations of the current gold standard surgical biopsy. The time consuming and costly biopsy procedure requires the inspection of each sample by a trained pathologist, which limits the analysis of potentially malignant lesions. Optical technologies like RAMAN or infrared spectroscopy, which provide both, localization and chemical information, can be used to differentiate malignant from healthy tissue by the analysis of multi cell structures and cell type specific spectra. We here report the application of midIR spectroscopy towards fast and reliable skin diagnostics. Within the European research project MINERVA we developed standardized in vitro skin systems with increasing complexity, from single skin cell types as fibroblasts, keratinocytes and melanoma cells, to mixtures of these and finally three dimensional human skin equivalents. The standards were characterized in the established midIR range and also with newly developed systems for fast imaging up to 12 μm. The analysis of the spectra by novel data processing algorithms demonstrated the clear separation of all cell types, especially the tumor cells. The signals from single cell layers were sufficient for cell type differentiation. We have compared different midIR systems and found all of them suitable for specific cell type identification. Our data demonstrate the potential of midIR spectroscopy for fast image acquisition and an improved data processing as sensitive and specific optical biopsy technology.

Paper Details

Date Published: 7 March 2016
PDF: 8 pages
Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 970307 (7 March 2016); doi: 10.1117/12.2208897
Show Author Affiliations
Lena Kastl, Univ. Muenster (Germany)
Björn Kemper, Univ. Muenster (Germany)
Gavin R. Lloyd, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Jayakrupakar Nallala, Exeter Univ. (United Kingdom)
Nick Stone, Exeter Univ. (United Kingdom)
Valery Naranjo, Univ. Politècnica de València (Spain)
Francisco Penaranda, Univ. Politècnica de València (Spain)
Jürgen Schnekenburger, Univ. Muenster (Germany)

Published in SPIE Proceedings Vol. 9703:
Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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