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Proceedings Paper

STED-like resolution enhancement with focus extension
Author(s): Kai-Ping Yang; Wei-Kuan Lin; Kuo-Jen Hsu; Yen-Yin Lin; Shi-Wei Chu
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Paper Abstract

In recent years, the techniques of super-resolution have generated widespread impacts in science. Stimulated emission depletion (STED) microscopy is known for achieving sub-diffraction-limit resolution by using a donut-shaped beam to deplete the fluorescence around a focal spot while leaving a central part active to emit fluorescence. However, since STED microscopy is based on fluorescence, it suffers from photo-bleaching. We recently developed a new technique and termed it as suppression of scattering imaging (SUSI) microscopy. It uses a STED-like setup and achieves super resolution imaging by utilizing the nonlinearity of scattering from gold nanoparticles. Therefore, SUSI microscopy avoids the photo-bleaching issue. Nonetheless, for fast volumetric imaging, SUSI microscopy is limited with slow axial translation of the objective or sample. Here we combine SUSI microscopy with a refractive-index-variable lens to axially move the focus at very high speed. This combination allows simultaneous observation of tissue dynamics over a three-dimensional volume within one second. The new technique paves the way toward high-speed super-resolution imaging for biological tissues.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97131N (9 March 2016); doi: 10.1117/12.2208865
Show Author Affiliations
Kai-Ping Yang, National Taiwan Univ. (Taiwan)
Wei-Kuan Lin, National Taiwan Univ. (Taiwan)
Kuo-Jen Hsu, National Taiwan Univ. (Taiwan)
Yen-Yin Lin, National Tsing Hua Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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