Share Email Print

Proceedings Paper

STED-like resolution enhancement with focus extension
Author(s): Kai-Ping Yang; Wei-Kuan Lin; Kuo-Jen Hsu; Yen-Yin Lin; Shi-Wei Chu
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In recent years, the techniques of super-resolution have generated widespread impacts in science. Stimulated emission depletion (STED) microscopy is known for achieving sub-diffraction-limit resolution by using a donut-shaped beam to deplete the fluorescence around a focal spot while leaving a central part active to emit fluorescence. However, since STED microscopy is based on fluorescence, it suffers from photo-bleaching. We recently developed a new technique and termed it as suppression of scattering imaging (SUSI) microscopy. It uses a STED-like setup and achieves super resolution imaging by utilizing the nonlinearity of scattering from gold nanoparticles. Therefore, SUSI microscopy avoids the photo-bleaching issue. Nonetheless, for fast volumetric imaging, SUSI microscopy is limited with slow axial translation of the objective or sample. Here we combine SUSI microscopy with a refractive-index-variable lens to axially move the focus at very high speed. This combination allows simultaneous observation of tissue dynamics over a three-dimensional volume within one second. The new technique paves the way toward high-speed super-resolution imaging for biological tissues.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97131N (9 March 2016); doi: 10.1117/12.2208865
Show Author Affiliations
Kai-Ping Yang, National Taiwan Univ. (Taiwan)
Wei-Kuan Lin, National Taiwan Univ. (Taiwan)
Kuo-Jen Hsu, National Taiwan Univ. (Taiwan)
Yen-Yin Lin, National Tsing Hua Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

© SPIE. Terms of Use
Back to Top