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Proceedings Paper

Single DNA imaging and length quantification through a mobile phone microscope
Author(s): Qingshan Wei; Wei Luo; Samuel Chiang; Tara Kappel; Crystal Mejia; Derek Tseng; Raymond Yan Lok Chan; Eddie Yan; Hangfei Qi; Faizan Shabbir; Haydar Ozkan; Steve Feng; Aydogan Ozcan
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Paper Abstract

The development of sensitive optical microscopy methods for the detection of single DNA molecules has become an active research area which cultivates various promising applications including point-of-care (POC) genetic testing and diagnostics. Direct visualization of individual DNA molecules usually relies on sophisticated optical microscopes that are mostly available in well-equipped laboratories. For POC DNA testing/detection, there is an increasing need for the development of new single DNA imaging and sensing methods that are field-portable, cost-effective, and accessible for diagnostic applications in resource-limited or field-settings. For this aim, we developed a mobile-phone integrated fluorescence microscopy platform that allows imaging and sizing of single DNA molecules that are stretched on a chip. This handheld device contains an opto-mechanical attachment integrated onto a smartphone camera module, which creates a high signal-to-noise ratio dark-field imaging condition by using an oblique illumination/excitation configuration. Using this device, we demonstrated imaging of individual linearly stretched λ DNA molecules (48 kilobase-pair, kbp) over 2 mm2 field-of-view. We further developed a robust computational algorithm and a smartphone app that allowed the users to quickly quantify the length of each DNA fragment imaged using this mobile interface. The cellphone based device was tested by five different DNA samples (5, 10, 20, 40, and 48 kbp), and a sizing accuracy of <1 kbp was demonstrated for DNA strands longer than 10 kbp. This mobile DNA imaging and sizing platform can be very useful for various diagnostic applications including the detection of disease-specific genes and quantification of copy-number-variations at POC settings.

Paper Details

Date Published: 7 March 2016
PDF: 7 pages
Proc. SPIE 9699, Optics and Biophotonics in Low-Resource Settings II, 969903 (7 March 2016); doi: 10.1117/12.2208836
Show Author Affiliations
Qingshan Wei, Univ. of California, Los Angeles (United States)
Wei Luo, Univ. of California, Los Angeles (United States)
Samuel Chiang, Univ. of California, Los Angeles (United States)
Tara Kappel, Univ. of California, Los Angeles (United States)
Crystal Mejia, Univ. of California, Los Angeles (United States)
Derek Tseng, Univ. of California, Los Angeles (United States)
Raymond Yan Lok Chan, Univ. of California, Los Angeles (United States)
Eddie Yan, Univ. of California, Los Angeles (United States)
Hangfei Qi, Univ. of California, Los Angeles (United States)
Faizan Shabbir, Univ. of California, Los Angeles (United States)
Haydar Ozkan, Univ. of California, Los Angeles (United States)
Steve Feng, Univ. of California, Los Angeles (United States)
Aydogan Ozcan, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 9699:
Optics and Biophotonics in Low-Resource Settings II
David Levitz; Aydogan Ozcan; David Erickson, Editor(s)

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