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Proceedings Paper

Characterization of human arterial tissue affected by atherosclerosis using multimodal nonlinear optical microscopy
Author(s): Enrico Baria; Riccardo Cicchi; Matteo Rotellini; Gabriella Nesi; Daniela Massi; Francesco S. Pavone
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Paper Abstract

Atherosclerosis is a widespread cardiovascular disease caused by the deposition of lipids (such as cholesterol and triglycerides) on the inner arterial wall. The rupture of an atherosclerotic plaque, resulting in a thrombus, is one of the leading causes of death in the Western World. Preventive assessment of plaque vulnerability is therefore extremely important and can be performed by studying collagen organization and lipid composition in atherosclerotic arterial tissues. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires immune-histochemical examination and a morpho-functional approach. Instead, a label-free and non-invasive alternative is provided by nonlinear microscopy. In this study, we combined SHG and FLIM microscopy in order to characterize collagen organization and lipids in human carotid ex vivo tissues affected by atherosclerosis. SHG and TPF images, acquired from different regions within atherosclerotic plaques, were processed through image pattern analysis methods (FFT, GLCM). The resulting information on collagen and cholesterol distribution and anisotropy, combined with collagen and lipids fluorescence lifetime measured from FLIM images, allowed characterization of carotid samples and discrimination of different tissue regions. The presented method can be applied for automated classification of atherosclerotic lesions and plaque vulnerability. Moreover, it lays the foundation for a potential in vivo diagnostic tool to be used in clinical setting.

Paper Details

Date Published: 14 March 2016
PDF: 6 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971217 (14 March 2016); doi: 10.1117/12.2208660
Show Author Affiliations
Enrico Baria, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
Riccardo Cicchi, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
National Institute of Optics, CNR (Italy)
Matteo Rotellini, Univ. of Florence (Italy)
Gabriella Nesi, Univ. of Florence (Italy)
Daniela Massi, Univ. of Florence (Italy)
Francesco S. Pavone, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
National Institute of Optics, CNR (Italy)


Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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