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Proceedings Paper

Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy
Author(s): Marcelina Cardoso Dos Santos; Cyrille Vézy; Rodolphe Jaffiol
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Paper Abstract

We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 97190I (9 March 2016); doi: 10.1117/12.2208429
Show Author Affiliations
Marcelina Cardoso Dos Santos, Lab. de Nanotechnologie et d'Instrumentation Optique, Institut Charles Delaunay, CNRS (France)
Univ. de Technologie de Troyes (France)
Cyrille Vézy, Lab. de Nanotechnologie et d'Instrumentation Optique, Institut Charles Delaunay, CNRS (France)
Univ. de Technologie de Troyes (France)
Rodolphe Jaffiol, Lab. de Nanotechnologie et d'Instrumentation Optique, Institut Charles Delaunay, CNRS (France)
Univ. de Technologie de Troyes (France)


Published in SPIE Proceedings Vol. 9719:
Biophysics, Biology, and Biophotonics: the Crossroads
Adam Wax; Vadim Backman, Editor(s)

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