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Proceedings Paper

Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar gold arrays
Author(s): Carlo Morasso; Silvia Picciolini; Dora Mehn; Paola Pellacani; Ana Frangolho; Gerardo Marchesini; Renzo Vanna; Alice Gualerzi; Marzia Bedoni; Franco Marabelli; Furio Gramatica
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Paper Abstract

The detection of biomarkers by means of Surface Enhanced Raman Spectroscopy (SERS) is foreseen to became a very important tool in the clinical practice because of its excellent sensitivity and potential for the simultaneous detection of multiple biomarkers. In the present paper we describe how it was possible to build a sensor for the detection of genetic biomarkers involved in acute myeloid leukemia. The assay is based on the use of a specifically designed SERS substrate made of a 2D crystal structure of polymeric pillars embedded in a gold layer. This substrate is characterized by good enhancing properties coupled with an excellent homogeneity. The SERS substrate was conjugated with DNA probes complementary to a target sequence and used in a sandwich assay with gold nanoparticles labeled with a second DNA probe and a Raman reporter. The so developed assay allowed the detection of a leukemia biomarker (WT1 gene) and an housekeeping gene with low picomolar sensitivity. At last, we optimized the assay in order to tackle one of the main limitations of SERS based assay: the loss of signal that is observed when the Raman spectra are collected in liquid. Combining a preferential functionalization on the polymeric pillars with a different height of the polymer pillars from the gold layer the assay demonstrated its effectiveness even when measured in buffer.

Paper Details

Date Published: 22 April 2016
PDF: 8 pages
Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 972404 (22 April 2016); doi: 10.1117/12.2208339
Show Author Affiliations
Carlo Morasso, Fondazione Don Carlo Gnocchi (Italy)
Silvia Picciolini, Fondazione Don Carlo Gnocchi (Italy)
Dora Mehn, Fondazione Don Carlo Gnocchi (Italy)
Paola Pellacani, Plasmore s.r.l. (Italy)
Ana Frangolho, Plasmore s.r.l. (Italy)
Gerardo Marchesini, BioSyPher (United Kingdom)
Renzo Vanna, Fondazione Don Carlo Gnocchi (Italy)
Alice Gualerzi, Fondazione Don Carlo Gnocchi (Italy)
Marzia Bedoni, Fondazione Don Carlo Gnocchi (Italy)
Franco Marabelli, Univ. of Pavia (Italy)
Furio Gramatica, Fondazione Don Carlo Gnocchi (Italy)

Published in SPIE Proceedings Vol. 9724:
Plasmonics in Biology and Medicine XIII
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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