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Proceedings Paper

Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues
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Paper Abstract

Second harmonic generation (SHG) is a powerful technique to observe fibrillar collagen without any staining and with a good contrast. More information about the molecular structure of collagen fibrils in tissues and their 3D distribution can be gained with polarization-resolved SHG imaging. Nevertheless, strong focusing is required for effective imaging and light propagation in tissues is complex and not thoroughly understood yet, preventing accurate and reproducible measurements. Theoretical analysis, vectorial numerical simulations and experiments were implemented to understand how the SHG signal builds up and how geometrical parameters affect polarization-resolved measurements in homogeneous collagen-rich tissues.

Paper Details

Date Published: 14 March 2016
PDF: 7 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971218 (14 March 2016); doi: 10.1117/12.2208110
Show Author Affiliations
Claire Teulon, LOB, Ecole Polytechnique, Inserm, Univ. Paris-Saclay, CNRS (France)
Ivan Gusachenko, LOB, Ecole Polytechnique, Inserm, Univ. Paris-Saclay, CNRS (France)
Okinawa Institute of Science and Technology (Japan)
Gaël Latour, IMNC, Univ. Paris-Sud, Univ. Paris-Diderot, Univ. Paris-Saclay, CNRS (France)
Marie-Claire Schanne-Klein, LOB, Ecole Polytechnique, Inserm, Univ. Paris-Saclay, CNRS (France)


Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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