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Proceedings Paper

Extremely sensitive dual imaging system in solid phantoms
Author(s): Eran A. Barnoy; Dror Fixler; Rachela Popovtzer; Tsviya Nayhoz; Krishanu Ray
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Paper Abstract

Herein we describe promising results from the combination of fluorescent lifetime imaging microscopy (FLIM) and diffusion reflection (DR) medical imaging techniques. Three different geometries of gold nanoparticles (GNPs) were prepared: spheres of 20nm diameter, rods (GNRs) of aspect ratio (AR) 2.5, and GNRs of AR 3.3. Each GNP geometry was then conjugated using PEG linkers estimated to be 10nm in length to each of 3 different fluorescent dyes: Fluorescein, Rhodamine B, and Sulforhodamine B. DR provided deep-volume measurements (up to 1cm) from within solid, tissue-imitating phantoms, indicating GNR presence corresponding to the light used by recording light scattered from the GNPs with increasing distance to a photodetector. FLIM imaged solutions as well as phantom surfaces, recording both the fluorescence lifetimes as well as the fluorescence intensities. Fluorescence quenching was observed for Fluorescein, while metal-enhanced fluorescence (MEF) was observed in Rhodamine B and Sulforhodamine B – the dyes with an absorption peak at a slightly longer wavelength than the GNP plasmon resonance peak. Our system is highly sensitive due to the increased intensity provided by MEF, and also because of the inherent sensitivity of both FLIM and DR. Together, these two modalities and MEF can provide a lot of meaningful information for molecular and functional imaging of biological samples.

Paper Details

Date Published: 22 April 2016
PDF: 7 pages
Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 972106 (22 April 2016); doi: 10.1117/12.2207908
Show Author Affiliations
Eran A. Barnoy, Bar-Ilan Univ. (Israel)
Dror Fixler, Bar-Ilan Univ. (Israel)
Rachela Popovtzer, Bar-Ilan Univ. (Israel)
Tsviya Nayhoz, Bar-Ilan Univ. (Israel)
Krishanu Ray, Univ. of Maryland School of Medicine (United States)

Published in SPIE Proceedings Vol. 9721:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Alexander N. Cartwright; Dan V. Nicolau; Dror Fixler, Editor(s)

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