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Proceedings Paper

Medical diagnosis and remote sensing at fiber-tip: picosecond resolved FRET sensor
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Paper Abstract

Förster Resonance Energy Transfer (FRET) strategy in popular in fiber-optic sensing. However, the steady state emission quenching of the donor is inadequate to conclude FRET. The resonance type energy transfer from one molecule (donor) to other (acceptor) should meet few key properties including donor to acceptor energy migration in non-radiative way. In the present study, we have coupled the evanescent field of an optical fiber to the covalently attached donor (dansyl) molecules at the fiber tip. By using picosecond resolved time correlated single photon counting (TCSPC) we have demonstrated that dansyl at the fiber tip transfers energy to a well known DNA-intercalating dye ethidium. Our ultrafast detection scheme selectively distinguishes the probe (dansyl) emission from the intrinsic emission of the fiber. We have also used the setup for the remote sensing of the dielectric constant (polarity) of an environment. We have finally implemented the detection mechanism to detect an industrial synthetic dye methylene blue (MB) in water.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 970211 (7 March 2016); doi: 10.1117/12.2207588
Show Author Affiliations
Nabarun Polley, S.N. Bose National Ctr. for Basic Sciences (India)
Samir Kumar Pal, S.N. Bose National Ctr. for Basic Sciences (India)


Published in SPIE Proceedings Vol. 9702:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI
Israel Gannot, Editor(s)

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