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Proceedings Paper

Alkyne-tag Raman imaging of bio-active small molecules in live cells
Author(s): Jun Ando; Almar F. Palonpon; Hiroyuki Yamakoshi; Kosuke Dodo; Satoshi Kawata; Mikiko Sodeoka; Katsumasa Fujita
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Paper Abstract

Raman microscopy is useful for molecular imaging and analysis of biological specimens. Here, we used alkyne containing a carbon-carbon triple bond as a Raman tag for observing small molecules in live cells. Alkyne tags can maintain original properties of target molecules with providing high chemical specificity owing to its distinct peak in a Raman-silent window of biomolecules. For demonstrations, alkyne-tagged thymidine and coenzyme Q analogue in live cells were visualized with high-spatial resolution. We extended the application of alkyne-tag imaging to visualize cell organelles and specific lipid components in artificial monolayer membranes.

Paper Details

Date Published: 9 December 2015
PDF: 7 pages
Proc. SPIE 9792, Biophotonics Japan 2015, 979207 (9 December 2015); doi: 10.1117/12.2207554
Show Author Affiliations
Jun Ando, Osaka Univ. (Japan)
RIKEN (Japan)
Almar F. Palonpon, Osaka Univ. (Japan)
Hiroyuki Yamakoshi, RIKEN (Japan)
Kosuke Dodo, AMED-CREST, AMED (Japan)
RIKEN (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
Mikiko Sodeoka, AMED-CREST, AMED (Japan)
RIKEN (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 9792:
Biophotonics Japan 2015
Takashige Omatsu; Yoshio Hayasaki; Yusuke Ogura; Yasuyuki Ozeki; Seigo Ohno, Editor(s)

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