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Proceedings Paper

Novel contrast mechanism for label free super-resolution imaging
Author(s): Sergey A. Alexandrov; James McGrath; Hrebesh Subhash; Francesca Boccafoschi; Cinzia Giannini; Martin Leahy
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Paper Abstract

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form super-resolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

Paper Details

Date Published: 9 March 2016
PDF: 8 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971303 (9 March 2016); doi: 10.1117/12.2207458
Show Author Affiliations
Sergey A. Alexandrov, National Univ. of Ireland, Galway (Ireland)
James McGrath, National Univ. of Ireland, Galway (Ireland)
Hrebesh Subhash, National Univ. of Ireland, Galway (Ireland)
Francesca Boccafoschi, Univ. of Piemonte Orientale "A. Avogadro" (Italy)
Cinzia Giannini, Institute of Crystallography, CNR (Italy)
Martin Leahy, National Univ. of Ireland, Galway (Ireland)


Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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