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Proceedings Paper

Non-interferometric quantitative phase imaging of yeast cells
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Paper Abstract

Real-time imaging of live cells is quite difficult without the addition of external contrast agents. Various methods for quantitative phase imaging of living cells have been proposed like digital holographic microscopy and diffraction phase microscopy. In this paper, we report theoretical and experimental results of quantitative phase imaging of live yeast cells with nanometric precision using transport of intensity equations (TIE). We demonstrate nanometric depth sensitivity in imaging live yeast cells using this technique. This technique being noninterferometric, does not need any coherent light sources and images can be captured through a regular bright-field microscope. This real-time imaging technique would deliver the depth or 3-D volume information of cells and is highly promising in real-time digital pathology applications, screening of pathogens and staging of diseases like malaria as it does not need any preprocessing of samples.

Paper Details

Date Published: 9 December 2015
PDF: 4 pages
Proc. SPIE 9792, Biophotonics Japan 2015, 97920G (9 December 2015); doi: 10.1117/12.2207232
Show Author Affiliations
Praveen Kumar Poola, Indian Institute of Technology Hyderabad (India)
Vimal Prabhu Pandiyan, Indian Institute of Technology Hyderabad (India)
Renu John, Indian Institute of Technology Hyderabad (India)

Published in SPIE Proceedings Vol. 9792:
Biophotonics Japan 2015
Takashige Omatsu; Yoshio Hayasaki; Yusuke Ogura; Yasuyuki Ozeki; Seigo Ohno, Editor(s)

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