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Proceedings Paper

Research of the interaction between kangai injection and human serum albumin by fluorescence spectroscopy
Author(s): Changbin Ye; Xiaogang Lin; Hao Zhu; Wenchao Li; Jie Wu
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Paper Abstract

The interaction between drugs and serum albumin is the theoretical basis of pharmacology research. Kangai injection with invigorating Qi, enhancing the immune function, is widely used for a variety of malignant tumor treatment. Fluorescence spectroscopy was adopted due to its high sensitivity and other advantages. The interaction between kangai injection and human serum albumin (HSA) in physiological buffer (pH 7.4) was investigated by fluorescence spectroscopy and UV-Vis absorption spectroscopy. The results of fluorescence spectrum at three temperature (296K, 303K and 310K) showed the degree of binding at 310K is the highest. Also, the maximum emission peak has a slight blue shift, which indicates that the interaction between kangai injection and HSA has an effect on the conformation of HSA. That is, the microenvironment of tryptophan increase hydrophobic due to the increase of the concentration of kangai injection. Results obtained from analysis of fluorescence spectrum and fluorescence intensity indicated that kangai injection has a strong ability to quench the intrinsic fluorescence of HSA. And according to the Stern-Volume equation, the quenching mechanism is static quenching, which is further proved by the UV-Vis absorption spectroscopy.

Paper Details

Date Published: 15 October 2015
PDF: 6 pages
Proc. SPIE 9672, AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications, 96720V (15 October 2015); doi: 10.1117/12.2202788
Show Author Affiliations
Changbin Ye, Chongqing Univ. (China)
Xiaogang Lin, Chongqing Univ. (China)
Hao Zhu, Chongqing Univ. (China)
Wenchao Li, Chongqing Univ. (China)
Jie Wu, The Univ. of Tennessee (United States)


Published in SPIE Proceedings Vol. 9672:
AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications
Byoungho Lee; Yikai Su; Min Gu; Xiaocong Yuan; Daniel Jaque, Editor(s)

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