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Proceedings Paper

Automation of daphtoxkit-F biotest using a microfluidic lab-on-a-chip technology
Author(s): Yushi Huang; Dayanthi Nugegoda; Donald Wlodkowic
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Paper Abstract

An increased rigor in water quality monitoring is not only a legal requirement, but is also critical to ensure timely chemical hazard emergency responses and protection of human and animal health. Bioindication is a method that applies very sensitive living organisms to detect environmental changes using their natural responses. Although bioindicators do not deliver information on an exact type or intensity of toxicants present in water samples, they do provide an overall snapshot and early-warning information about presence of harmful and dangerous parameters. Despite the advantages of biotests performed on sentinel organisms, their wider application is limited by the nonexistence of high-throughput laboratory automation systems. As a result majority of biotests used in ecotoxicology require time-consuming and laborious manual procedures.

In this work, we present development of a miniaturized Lab-on-a-Chip (LOC) platform for automation and enhancement of acute ecotoxicity test based on immobilization of a freshwater crustacean Daphnia magna (Daphtoxkit-FTM). Daphnids’ immobilization in response to sudden changes in environment parameters is fast, unambiguous, and easy to record optically. We also for the first time demonstrate that LOC system enables studies of sub-lethal ecotoxic effects using behavioral responses of Daphnia magna as sentinels of water pollution.

The system working principle incorporated a high definition (HD) time-resolved video data analysis to dynamically assess impact of the reference toxicant on swimming behavior of D. magna. Our system design combined: (i) microfluidic device for caging of Daphnia sp.; (ii) mechatronic interface for fluidic actuation; (iii) video data acquisition; and (iv) algorithms for animal movement tracking and analysis.

Paper Details

Date Published: 22 December 2015
PDF: 7 pages
Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 966813 (22 December 2015); doi: 10.1117/12.2202396
Show Author Affiliations
Yushi Huang, RMIT Univ. (Australia)
Dayanthi Nugegoda, RMIT Univ. (Australia)
Donald Wlodkowic, RMIT Univ. (Australia)


Published in SPIE Proceedings Vol. 9668:
Micro+Nano Materials, Devices, and Systems
Benjamin J. Eggleton; Stefano Palomba, Editor(s)

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