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Proceedings Paper

A homeostatic, chip-based platform for zebrafish larvae immobilization and long-term imaging
Author(s): Timo Friedrich; Feng Zhu; Donald Wlodkowic; Jan Kaslin
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Paper Abstract

Zebrafish larvae are ideal for toxicology and drug screens due to their transparency, small size and similarity to humans on the genetic level. Using modern imaging techniques, cells and tissues can be dynamically visualised and followed over days in multiple zebrafish. Yet continued imaging experiments require specialized conditions such as: moisture and heat control to maintain specimen homeostasis. Chambers that control the environment are generally very expensive and are not always available for all imaging platforms. A highly customizable mounting configuration with built-in means of controlling temperature and media flow would therefore be a valuable tool for long term imaging experiments. Rapid prototyping using 3D printing is particularly suitable as a production method as it offers high flexibility in design, is widely available and allows a high degree of customizing. We study neural regeneration in zebrafish. Regeneration is limited in humans, but zebrafish recover from neural damage within days. Yet, the underlying regenerative mechanisms remain unclear.

We developed an agarose based mounting system that holds the embryos in defined positions along removable strips. Homeostasis and temperature control is ensured by channels circulating buffer and heated water. This allows to image up to 120 larvae simultaneously for more than two days. Its flexibility and the low-volume, high larvae ratio will allow screening of small compound libraries. Taken together, we offer a low cost, highly adaptable solution for long term in-vivo imaging.

Paper Details

Date Published: 22 December 2015
PDF: 10 pages
Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 96682Y (22 December 2015); doi: 10.1117/12.2202390
Show Author Affiliations
Timo Friedrich, Australian Regenerative Medicine Institute (Australia)
Feng Zhu, RMIT Univ. (Australia)
Donald Wlodkowic, RMIT Univ. (Australia)
Jan Kaslin, Australian Regenerative Medicine Institute (Australia)

Published in SPIE Proceedings Vol. 9668:
Micro+Nano Materials, Devices, and Systems
Benjamin J. Eggleton; Stefano Palomba, Editor(s)

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