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Proceedings Paper

Liquid marble as microbioreactor for bioengineering applications
Author(s): Fatemeh Sarvi; Kanika Jain; Layla Alhasan; Tina Arbatan; Wei Shen; Peggy P. Y. Chan
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Paper Abstract

This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.

Paper Details

Date Published: 22 December 2015
PDF: 6 pages
Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 966852 (22 December 2015); doi: 10.1117/12.2201110
Show Author Affiliations
Fatemeh Sarvi, Monash Univ. (Australia)
Kanika Jain, Monash Univ. (Australia)
Layla Alhasan, RMIT Univ. (Australia)
Tina Arbatan, Monash Univ. (Australia)
Wei Shen, Monash Univ. (Australia)
Peggy P. Y. Chan, Swinburne Univ. of Technology (Australia)


Published in SPIE Proceedings Vol. 9668:
Micro+Nano Materials, Devices, and Systems
Benjamin J. Eggleton; Stefano Palomba, Editor(s)

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