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Proceedings Paper

Dynamic speckle study of microbial growth
Author(s): F. M. Vincitorio; C. Mulone; P. A. Marcuzzi; N. Budini; C. Freyre; A. J. Lopez; A. Ramil
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Paper Abstract

In this work we present a characterization of yeast dynamic speckle activity during growth in an isolated agar culture medium. We found that it is possible to detect the growth of the microorganisms even before they turn out to be visible. By observing the time evolution of the speckle activity at different regions of the culture medium we could extract a map of the growth process, which served to analyze how the yeast develops and spreads over the agar's medium. An interesting point of this study concerns with the influence of the laser light on the yeast growth rate. We have found that yeast finds hard to develop at regions with higher laser light illumination, although we used a synchronous system to capture the speckle pattern. The results obtained in this work would serve us as a starting point to fabricate a detector of growing microorganism colonies, with obvious interesting applications in diverse areas.

Paper Details

Date Published: 24 August 2015
PDF: 6 pages
Proc. SPIE 9660, SPECKLE 2015: VI International Conference on Speckle Metrology, 96601Q (24 August 2015); doi: 10.1117/12.2196257
Show Author Affiliations
F. M. Vincitorio, Univ. Tecnologica Nacional (Argentina)
C. Mulone, Univ. Tecnologica Nacional (Argentina)
P. A. Marcuzzi, Univ. Tecnológica Nacional (Argentina)
N. Budini, Univ. Tecnologica Nacional (Argentina)
Instituto de Física del Litoral (UNL-CONICET) (Argentina)
C. Freyre, Univ. Tecnológica Nacional (Argentina)
A. J. Lopez, Univ. da Coruña (Spain)
A. Ramil, Univ. da Coruña (Spain)

Published in SPIE Proceedings Vol. 9660:
SPECKLE 2015: VI International Conference on Speckle Metrology
Fernando Mendoza Santoyo; Eugenio R. Mendez, Editor(s)

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