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Proceedings Paper

Application of dynamic speckle interferometry for diagnosis of metabolism change in cultured cells
Author(s): A. P. Vladimirov; J. A. Mikhailova; A. A. Baharev; A. S. Malygin; I. A. Novosyolova; D. I. Yakin
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Paper Abstract

Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities. The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. Defrosted L-41 cells precipitated on a glass substrate were the target of the research. Time-average value T of digital radiation intensity in the TV camera pixels I~ (1) and the correlation coefficient of two digital images η (2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments.

Paper Details

Date Published: 24 August 2015
PDF: 10 pages
Proc. SPIE 9660, SPECKLE 2015: VI International Conference on Speckle Metrology, 96601M (24 August 2015); doi: 10.1117/12.2196090
Show Author Affiliations
A. P. Vladimirov, Ural Federal Univ. (Russian Federation)
Yekaterinburg Research Institute of Viral Infections (Russian Federation)
J. A. Mikhailova, Ural Federal Univ. (Russian Federation)
Yekaterinburg Research Institute of Viral Infections (Russian Federation)
A. A. Baharev, Yekaterinburg Research Institute of Viral Infections (Russian Federation)
A. S. Malygin, Ural Federal Univ. (Russian Federation)
I. A. Novosyolova, Ural Federal Univ. (Russian Federation)
D. I. Yakin, Ural Federal Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 9660:
SPECKLE 2015: VI International Conference on Speckle Metrology
Fernando Mendoza Santoyo; Eugenio R. Mendez, Editor(s)

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