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Proceedings Paper

Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors
Author(s): Diego Voccia; Francesca Bettazzi; Ilaria Palchetti
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Paper Abstract

In recent years various kinds of biosensors for the detection of pathogens have been developed. A genosensor consists in the immobilization, onto the surface of a chosen transducer, of an oligonucleotide with a specific base sequence called capture probe. The complementary sequence (the analytical target, i.e. a specific sequence of the DNA/RNA of the pathogen) present in the sample is recognized and captured by the probe through the hybridization reaction. The evaluation of the extent of the hybridization allows one to confirm whether the sample contains the complementary sequence of the probe or not. Electrochemical transducers have received considerable attention in connection with the detection of DNA hybridization. Moreover, recently, with the emergence of novel photoelectrochemically active species and new detection schemes, photoelectrochemistry has resulted in substantial progress in its analytical performance for biosensing applications. In this paper, some examples of electrochemical genosensors for multiplexed pathogen detection are shown. Moreover, the preliminary experiments towards the development of a photoelectrochemical genosensor using a TiO2 – nanocrystal-modified ITO electrode are discussed.

Paper Details

Date Published: 21 October 2015
PDF: 9 pages
Proc. SPIE 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII, 96520T (21 October 2015); doi: 10.1117/12.2194838
Show Author Affiliations
Diego Voccia, Univ. of Florence (Italy)
Francesca Bettazzi, Univ. of Florence (Italy)
Ilaria Palchetti, Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 9652:
Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII
Roberto Zamboni; Douglas Burgess; Gari Owen; François Kajzar; Attila A. Szep; Harbinder Rana, Editor(s)

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