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Proceedings Paper

Assessment of dental plaque by optoelectronic methods
Author(s): Meda-Lavinia Negrutiu; Cosmin Sinescu; Cristina Maria Bortun; Mihaela-Codrina Levai; Florin Ionel Topala; Emanuela Lidia Crăciunescu; Andreea Codruta Cojocariu; Virgil Florin Duma; Adrian Gh. Podoleanu
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Paper Abstract

The formation of dental biofilm follows specific mechanisms of initial colonization on the surface, microcolony formation, development of organized three dimensional community structures, and detachment from the surface. The structure of the plaque biofilm might restrict the penetration of antimicrobial agents, while bacteria on a surface grow slowly and display a novel phenotype; the consequence of the latter is a reduced sensitivity to inhibitors. The aim of this study was to evaluate with different optoelectronic methods the morphological characteristics of the dental biofilm. The study was performed on samples from 25 patients aged between 18 and 35 years. The methods used in this study were Spectral Domain Optical Coherence Tomography (SD-OCT) working at 870 nm for in vivo evaluations and Scanning Electron Microscopy (SEM) for validations. For each patient a sample of dental biofilm was obtained directly from the vestibular surface of the teeth's. SD-OCT produced C- and B-scans that were used to generate three dimensional (3D) reconstructions of the sample. The results were compared with SEM evaluations. The biofilm network was dramatically destroyed after the professional dental cleaning. OCT noninvasive methods can act as a valuable tool for the 3D characterization of dental biofilms.

Paper Details

Date Published: 22 March 2016
PDF: 9 pages
Proc. SPIE 9670, Sixth International Conference on Lasers in Medicine, 96700W (22 March 2016); doi: 10.1117/12.2191640
Show Author Affiliations
Meda-Lavinia Negrutiu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Cosmin Sinescu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Cristina Maria Bortun, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Mihaela-Codrina Levai, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Florin Ionel Topala, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Emanuela Lidia Crăciunescu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Andreea Codruta Cojocariu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Virgil Florin Duma, Aurel Vlaicu Univ. of Arad (Romania)
Politehnica Univ. of Timisoara (Romania)
Adrian Gh. Podoleanu, Univ. of Kent (United Kingdom)


Published in SPIE Proceedings Vol. 9670:
Sixth International Conference on Lasers in Medicine
Darinca Carmen Todea; Adrian Gh. Podoleanu; Virgil-Florin Duma, Editor(s)

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