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Proceedings Paper

Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)
Author(s): Samira Aghayee; Dan Winkowski; Patrick Kanold; Wolfgang Losert
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Paper Abstract

The spatial connectivity of neural circuits and the various activity patterns they exert is what forms the brain function. How these patterns link to a certain perception or a behavior is a key question in neuroscience. Recording the activity of neural circuits while manipulating arbitrary neurons leads to answering this question. That is why acquiring a fast and reliable method of stimulation and imaging a population of neurons at a single cell resolution is of great importance. Owing to the recent advancements in calcium imaging and optogenetics, tens to hundreds of neurons in a living system can be imaged and manipulated optically. We describe the adaptation of a multi-point optical method that can be used to address the specific challenges faced in the in-vivo study of neuronal networks in the cerebral cortex. One specific challenge in the cerebral cortex is that the information flows perpendicular to the surface. Therefore, addressing multiple points in a three dimensional space simultaneously is of great interest. Using a liquid crystal spatial light modulator, the wavefront of the input laser beam is modified to produce multiple focal points at different depths of the sample for true multipoint two-photon excitation.

Paper Details

Date Published: 5 October 2015
PDF: 1 pages
Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95481P (5 October 2015); doi: 10.1117/12.2191538
Show Author Affiliations
Samira Aghayee, Univ. of Maryland, College Park (United States)
Dan Winkowski, Univ. of Maryland, College Park (United States)
Patrick Kanold, Univ. of Maryland, College Park (United States)
Wolfgang Losert, Univ. of Maryland, College Park (United States)

Published in SPIE Proceedings Vol. 9548:
Optical Trapping and Optical Micromanipulation XII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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