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Proceedings Paper

Single-cell diffraction tomography with optofluidic rotation about a tilted axis
Author(s): Paul Müller; Mirjam Schürmann; Chii J. Chan; Jochen Guck
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Paper Abstract

Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be regarded as a generalization of optical projection tomography which is equivalent to computerized tomography (CT). Both optical tomographic techniques require projection-phase images of cells measured at multiple angles. However, the reconstruction of the 3D refractive index distribution post-measurement differs for the two techniques. It is known that ODT yields better results than projection tomography, because it takes into account diffraction of the imaging light due to the refractive index structure of the sample. Here, we apply ODT to biological cells in a microfluidic chip which combines optical trapping and microfluidic flow to achieve an optofluidic single-cell rotation. In particular, we address the problem that arises when the trapped cell is not rotating about an axis perpendicular to the imaging plane, but is instead arbitrarily tilted. In this paper we show that the 3D reconstruction can be improved by taking into account such a tilted rotational axis in the reconstruction process.

Paper Details

Date Published: 25 August 2015
PDF: 5 pages
Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95480U (25 August 2015); doi: 10.1117/12.2191501
Show Author Affiliations
Paul Müller, TU Dresden (Germany)
Mirjam Schürmann, TU Dresden (Germany)
Chii J. Chan, TU Dresden (Germany)
Jochen Guck, TU Dresden (Germany)

Published in SPIE Proceedings Vol. 9548:
Optical Trapping and Optical Micromanipulation XII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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