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Proceedings Paper

Artifacts from photobleaching and dye-dissociation in fluorescence correlation spectroscopy
Author(s): Rudra P. Kafle; Molly R. Liebeskind; Julia T. Bourg; Elizabeth White; Jens-Christian Meiners
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Paper Abstract

Fluorescence correlation spectroscopy (FCS) is an optical technique in which the fluctuations in fluorescence intensity are quantified. The time correlation function gives insight into the dynamics of the molecule in its environment: typically the diffusion coefficient in a dilute solution is measured, but the technique has been expanded to uses in more complex environments, including living cells. In these environments photobleaching and dye-dissociation can substantially introduce artifacts in the FCS data. We present a technique to correct for the artifacts introduced by photobleaching and study dye dissociation in DNA solutions.

Paper Details

Date Published: 25 August 2015
PDF: 8 pages
Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95481M (25 August 2015); doi: 10.1117/12.2191402
Show Author Affiliations
Rudra P. Kafle, Univ. of Michigan (United States)
Molly R. Liebeskind, Univ. of Michigan (United States)
Julia T. Bourg, Univ. of Michigan (United States)
Elizabeth White, Amherst College (United States)
Jens-Christian Meiners, Univ. of Michigan (United States)


Published in SPIE Proceedings Vol. 9548:
Optical Trapping and Optical Micromanipulation XII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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