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Proceedings Paper

Studies on shape anisotropy in red blood cells
Author(s): Basudev Roy; Argha Mondal; Sudipta Bera; Ayan Banerjee
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Paper Abstract

Asymmetric particles, such as biological cells, often experience torque under optical tweezers. The cause is believed to be either birefringence or unbalanced scattering forces. The estimate of torque relies on the accurate measurement of rotational motion. Here we present a new technique to quantify the asymmetry of trapped particles relying upon the cross coupling between rotational and translational Brownian motion. We observe that RBC does indeed show cross coupling indicating asymmetry of the shape. Further we also show by polarimetry that the retardance of the RBC is not sufficient to make it rotate since the scattering torque is much higher.

Paper Details

Date Published: 25 August 2015
PDF: 7 pages
Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95481Z (25 August 2015); doi: 10.1117/12.2191125
Show Author Affiliations
Basudev Roy, Indian Institute of Science Education and Research Kolkata (India)
Argha Mondal, Indian Institute of Science Education and Research Kolkata (India)
Sudipta Bera, Indian Institute of Science Education and Research Kolkata (India)
Ayan Banerjee, Indian Institute of Science Education and Research Kolkata (India)


Published in SPIE Proceedings Vol. 9548:
Optical Trapping and Optical Micromanipulation XII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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