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Proceedings Paper

High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion
Author(s): Xiang Lu; Rainer Heintzmann; Ulrich Leischner
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Paper Abstract

Light sheet microscopy is a microscopy technique characterized by an illumination from the side, perpendicular to the direction of observation. While this is often used and easy to implement for imaging samples with water-immersion, the application in combination with oil-immersion is less often used and requires a specific optimization. In this paper we present our design of a light-sheet illumination optical system with a ~1μm illumination thickness, a long working distance through the immersion oil, and including a focusing system allowing for moving the focus-spot of the lightsheet laterally through the field of view. This optical design allows for the acquisition of fluorescence images in 3D with isotropic resolution of below 1 micrometer of whole-mount samples with a size of ~1mm diameter. This technique enables high-resolution insights in the 3D structure of biological samples, e.g. for research of insect anatomy or for imaging of biopsies in medical diagnostics.

Paper Details

Date Published: 23 September 2015
PDF: 12 pages
Proc. SPIE 9626, Optical Systems Design 2015: Optical Design and Engineering VI, 96262I (23 September 2015); doi: 10.1117/12.2190823
Show Author Affiliations
Xiang Lu, Friedrich-Schiller-Univ. Jena (Germany)
Leibniz-Institut für Photonische Technologien e.V. (Germany)
Rainer Heintzmann, Friedrich-Schiller-Univ. Jena (Germany)
Leibniz-Institut für Photonische Technologien e.V. (Germany)
Ulrich Leischner, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Univ. Hospital Jena (Germany)


Published in SPIE Proceedings Vol. 9626:
Optical Systems Design 2015: Optical Design and Engineering VI
Laurent Mazuray; Rolf Wartmann; Andrew P. Wood, Editor(s)

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