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Proceedings Paper

Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers
Author(s): Jord C. Prangsma; Robert Molenaar; Vinod Subramaniam; Christian Blum
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Paper Abstract

In this paper we investigate the expected change in fluorescent decay rate when a fluorophore in aqueous solution is moved to a thin spin-coated layer of poly(vinyl alcohol). We take into account the local field effect due to the change in the refractive index of the medium around the fluorophore and the photonic effect due to the layers. The obtained results are compared with experimental results for the organic dye Atto565 and the fluorescent protein mCherry. We find that the effects for the organic dye can be well described with the model, for the fluorescent protein (FP) the model is less accurate. We discuss the possible explanations for this.

Paper Details

Date Published: 26 August 2015
PDF: 8 pages
Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95540G (26 August 2015); doi: 10.1117/12.2190521
Show Author Affiliations
Jord C. Prangsma, Univ. Twente (Netherlands)
Robert Molenaar, Univ. Twente (Netherlands)
Vinod Subramaniam, FOM Institute for Atomic and Molecular Physics (Netherlands)
Christian Blum, Univ. Twente (Netherlands)

Published in SPIE Proceedings Vol. 9554:
Nanoimaging and Nanospectroscopy III
Prabhat Verma; Alexander Egner, Editor(s)

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