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Proceedings Paper

Reactive oxygen species production in single cells following laser irradiation (Presentation Recording)
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Paper Abstract

Region specific DNA breaks can be created in single cells using laser light that damages DNA but does not directly generate reactive oxygen species (ROS). We have examined the cellular response to directly generated DNA breaks in single cells. Using a combination of ROS specific dyes and oxidase inhibitors we have found that the oxidase and chromatin remodeling protein Lysine demethylase I (LSD1) generates detectable ROS as a byproduct of its chromatin remodeling activity during the initial DNA damage response. ROS is produced at detectable amounts primarily within the first 3 minutes post irradiation. LSD1 activity has been previously associated with transcriptional regulation therefore these findings have implications for regulation of gene expression following DNA damage particularly in cells with altered redox states.

Paper Details

Date Published: 5 October 2015
PDF: 1 pages
Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95480S (5 October 2015); doi: 10.1117/12.2190375
Show Author Affiliations
Michelle L. Duquette, Univ. of California, San Diego (United States)
Justine Kim, Univ. of California, San Diego (United States)
Linda Z. Shi, Univ. of California, San Diego (United States)
Michael W. Berns, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 9548:
Optical Trapping and Optical Micromanipulation XII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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