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Proceedings Paper

Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA
Author(s): Inhong Kim; Mijeong Kang; Han Young Woo; Jin-Woo Oh; Kwangseuk Kyhm
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Paper Abstract

We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 Å) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 Å and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.

Paper Details

Date Published: 8 July 2015
PDF: 7 pages
Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230N (8 July 2015); doi: 10.1117/12.2190332
Show Author Affiliations
Inhong Kim, Pusan National Univ. (Korea, Republic of)
Mijeong Kang, KAIST (Korea, Republic of)
Han Young Woo, Pusan National Univ. (Korea, Republic of)
Jin-Woo Oh, Pusan National Univ. (Korea, Republic of)
Kwangseuk Kyhm, Pusan National Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 9523:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015
Donghyun Kim; Min-Gon Kim; Seung-Han Park, Editor(s)

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