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Proceedings Paper

Quantitative high spatiotemporal imaging of biological processes
Author(s): Joe Borbely; Jason Otterstrom; Nitin Mohan; Carlo Manzo; Melike Lakadamyali
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Paper Abstract

Super-resolution microscopy has revolutionized fluorescence imaging providing access to length scales that are much below the diffraction limit. The super-resolution methods have the potential for novel discoveries in biology. However, certain technical limitations must be overcome for this potential to be fulfilled. One of the main challenges is the use of super-resolution to study dynamic events in living cells. In addition, the ability to extract quantitative information from the super-resolution images is confounded by the complex photophysics that the fluorescent probes exhibit during the imaging. Here, we will review recent developments we have been implementing to overcome these challenges and introduce new steps in automated data acquisition towards high-throughput imaging.

Paper Details

Date Published: 26 August 2015
PDF: 9 pages
Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95540M (26 August 2015); doi: 10.1117/12.2190263
Show Author Affiliations
Joe Borbely, ICFO - Institut de Ciències Fotòniques (Spain)
Jason Otterstrom, ICFO - Institut de Ciències Fotòniques (Spain)
Nitin Mohan, ICFO - Institut de Ciències Fotòniques (Spain)
Carlo Manzo, ICFO - Institut de Ciències Fotòniques (Spain)
Melike Lakadamyali, ICFO - Institut de Ciències Fotòniques (Spain)


Published in SPIE Proceedings Vol. 9554:
Nanoimaging and Nanospectroscopy III
Prabhat Verma; Alexander Egner, Editor(s)

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