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Proceedings Paper

Sensitive and selective real-time electrochemical monitoring of DNA repair (Presentation Recording)
Author(s): Jason D. Slinker; Marc McWilliams; Fadwa Anka; Kenneth Balkus
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Paper Abstract

Unrepaired DNA damage can lead to mutation, cancer, and death of cells or organisms. However, due to the subtlety of DNA damage, it is difficult to sense the repair of damage products with high selectivity and sensitivity. Here, we show sensitive and selective electrochemical sensing of the repair activity of 8-oxoguanine and uracil glycosylases within DNA monolayers on gold by multiplexed analysis with silicon chips and low-cost electrospun nanofibers. Our approach involves comparing the electrochemical signal of redox probe modified monolayers containing the defect versus the rational control of defect-free monolayers. We find sequence-specific sensitivity thresholds on the order of femtomoles of proteins and dynamic ranges of over two orders of magnitude for each target. For 8-oxoguanine repair, temperature-dependent kinetics are extracted, showing exponential signal loss with time constants of seconds. Electrospun fibers are shown to behave similarly to conventional gold-on-silicon devices, showing the potential of these low-cost devices for sensing applications.

Paper Details

Date Published: 5 October 2015
PDF: 1 pages
Proc. SPIE 9568, Organic Field-Effect Transistors XIV; and Organic Sensors and Bioelectronics VIII, 95681K (5 October 2015); doi: 10.1117/12.2190226
Show Author Affiliations
Jason D. Slinker, The Univ. of Texas at Dallas (United States)
Marc McWilliams, The Univ. of Texas at Dallas (United States)
Fadwa Anka, The Univ. of Texas at Dallas (United States)
Kenneth Balkus, The Univ. of Texas at Dallas (United States)


Published in SPIE Proceedings Vol. 9568:
Organic Field-Effect Transistors XIV; and Organic Sensors and Bioelectronics VIII
Ioannis Kymissis; Iain McCulloch; Ruth Shinar; Oana D. Jurchescu; Luisa Torsi, Editor(s)

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