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Proceedings Paper

Fluorescence multi-scale endoscopy and its applications in the study and diagnosis of gastro-intestinal diseases: set-up design and software implementation
Author(s): Pablo Aurelio Gómez-García; Alicia Arranz; Manuel Fresno; Manuel Desco; Umar Mahmood; Juan José Vaquero; Jorge Ripoll
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Paper Abstract

Endoscopy is frequently used in the diagnosis of several gastro-intestinal pathologies as Crohn disease, ulcerative colitis or colorectal cancer. It has great potential as a non-invasive screening technique capable of detecting suspicious alterations in the intestinal mucosa, such as inflammatory processes. However, these early lesions usually cannot be detected with conventional endoscopes, due to lack of cellular detail and the absence of specific markers. Due to this lack of specificity, the development of new endoscopy technologies, which are able to show microscopic changes in the mucosa structure, are necessary. We here present a confocal endomicroscope, which in combination with a wide field fluorescence endoscope offers fast and specific macroscopic information through the use of activatable probes and a detailed analysis at cellular level of the possible altered tissue areas. This multi-modal and multi-scale imaging module, compatible with commercial endoscopes, combines near-infrared fluorescence (NIRF) measurements (enabling specific imaging of markers of disease and prognosis) and confocal endomicroscopy making use of a fiber bundle, providing a cellular level resolution. The system will be used in animal models exhibiting gastro-intestinal diseases in order to analyze the use of potential diagnostic markers in colorectal cancer. In this work, we present in detail the set-up design and the software implementation in order to obtain simultaneous RGB/NIRF measurements and short confocal scanning times.

Paper Details

Date Published: 19 June 2015
PDF: 10 pages
Proc. SPIE 9531, Biophotonics South America, 953111 (19 June 2015); doi: 10.1117/12.2189960
Show Author Affiliations
Pablo Aurelio Gómez-García, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria del Hospital Gregorio (Spain)
Alicia Arranz, CSIC (Spain)
Manuel Fresno, CSIC (Spain)
Manuel Desco, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria del Hospital Gregorio (Spain)
Umar Mahmood, Massachusetts General Hospital (United States)
Juan José Vaquero, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria del Hospital Gregorio (Spain)
Jorge Ripoll, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria del Hospital Gregorio (Spain)


Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi; Katarina Svanberg; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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