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Proceedings Paper

Monte Carlo simulations of triplet-state photophysics for super-resolution imaging of fluorophore-labeled gold nanorods
Author(s): Eric J. Titus; Karole L. Blythe; Katherine A. Willets
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Paper Abstract

Super-resolution imaging has previously been used to identify the position of individual fluorescently-labeled DNA molecules bound to the surface of gold nanorods. In order to isolate and fit emission from individual fluorophores, a stochastic photoswitching technique based on shelving the fluorophores into triplet states is used. However, the reconstructed super-resolution images of the fluorescently-labeled gold nanorods are consistently smaller than the expected size of the gold nanorod supports. Here, Monte Carlo simulations are used to probe how smaller-than-expected reconstructed images may be obtained by simultaneous emission events based on short triplet state lifetimes and/or a high density of fluorescently-labeled DNA on the gold nanorod surface.

Paper Details

Date Published: 26 August 2015
PDF: 10 pages
Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 955403 (26 August 2015); doi: 10.1117/12.2189926
Show Author Affiliations
Eric J. Titus, Univ. of Texas at Austin (United States)
Temple Univ. (United States)
Karole L. Blythe, Univ. of Texas at Austin (United States)
Temple Univ. (United States)
Katherine A. Willets, Univ. of Texas at Austin (United States)
Temple Univ. (United States)

Published in SPIE Proceedings Vol. 9554:
Nanoimaging and Nanospectroscopy III
Prabhat Verma; Alexander Egner, Editor(s)

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