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Proceedings Paper

Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection
Author(s): H. Chon; S. Lee; R. Wang; S.-Y. Bang; H.-S. Lee; S.-C. Bae; S. H. Hong; Y. H. Yoon; D. Lim; J. Choo
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Paper Abstract

We report a highly sensitive anti-cyclic citrullinated peptide (anti-CCP) detection method for early diagnosis of rheumatoid arthritis (RA) using surface-enhanced Raman scattering (SERS)-based immunoassay. Herein, cyclic citrullinated peptide (CCP)-conjugated magnetic beads and anti-human IgG-conjugated hollow gold nanospheres (HGNs) were used as substrates and SERS nano-tags, respectively. First, its detection sensitivity was evaluated using anti-CCP standard solutions. Then quantitative anti-CCP levels, determined by the SERS-based assay, were compared with those obtained from three commercially available anti-CCP assay kits (Immunoscan CCPlus, ImmunnLisa CCP and BioPlex 2200) to assess its potential utility as a clinical tool. Finally, clinical samples from 20 RA patients were investigated using them. In the SERS-based assay, the anti-CCP level in human serum was successfully determined by monitoring the characteristic Raman peak intensity of SERS nano-tags. The diagnostic performance of our SERS-based immunoassay for clinical samples shows a good agreement with those measured by three commercial anti-CCP kits. In addition, our SERS-based assay results are more consistent in the low concentration range (0–25 U/mL) than those achieved by the commercial kits. Accordingly, it is estimated that the SERS-based assay is a potentially useful diagnostic tool for early diagnosis of RA.

Paper Details

Date Published: 8 July 2015
PDF: 8 pages
Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230J (8 July 2015); doi: 10.1117/12.2189760
Show Author Affiliations
H. Chon, Hanyang Univ. (Korea, Republic of)
S. Lee, Hanyang Univ. (Korea, Republic of)
R. Wang, Hanyang Univ. (Korea, Republic of)
S.-Y. Bang, Hanyang Univ. Hospital for Rheumatic Diseases (Korea, Republic of)
H.-S. Lee, Hanyang Univ. Hospital for Rheumatic Diseases (Korea, Republic of)
S.-C. Bae, Hanyang Univ. Hospital for Rheumatic Diseases (Korea, Republic of)
S. H. Hong, EOne Labs. (Korea, Republic of)
Y. H. Yoon, EOne Labs. (Korea, Republic of)
D. Lim, Hanyang Univ. (Korea, Republic of)
J. Choo, Hanyang Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 9523:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015
Donghyun Kim; Min-Gon Kim; Seung-Han Park, Editor(s)

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