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Proceedings Paper

Observation of Brownian motion of a micrometer-size bead in water by off-axis digital holographic microscopy
Author(s): Yoon-Sung Bae; Phil-Jun Jeon; Hee-Jung Lee
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Paper Abstract

An effective volumetric measurement method for determination of the 3D position of a single particle based on off-axis digital holographic microscopy is presented in this paper. 3 μm polystyrene bead suspended water is used as a particle to be traced in our experiment. We have demonstrated the feasibility of our method by observing the bead undergoing Brownian motion in water by implemented setup. For fast determination, a series of transverse intensity images are numerically reconstructed from a single hologram of the bead, then centroid technique is applied to the reconstructed images for enhancing the position resolution. Nano-meter scale resolution with a frame rate of 30 is achieved in this experiment in both lateral and transverse direction.

Paper Details

Date Published: 8 July 2015
PDF: 5 pages
Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230A (8 July 2015); doi: 10.1117/12.2189433
Show Author Affiliations
Yoon-Sung Bae, Gwangju Institute of Science and Technology (Korea, Republic of)
Phil-Jun Jeon, Yonsei Univ. (Korea, Republic of)
Hee-Jung Lee, Yonsei Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 9523:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015
Donghyun Kim; Min-Gon Kim; Seung-Han Park, Editor(s)

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